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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human FAAP100
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human FAAP100 ELISA KIT
Product Name:Human FAAP100 ELISA KIT
Packing:96T
Catalog No.:ELI-44072h
Gene Name:FAAP100
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Human FAAP100
Alternative Name:FAAP100,Human FAAP100
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human FAAP100 ELISA KIT allows for the in vitro quantitative determination of Human FAAP100 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human FAAP100 ELISA KIT has been pre-coated with an Human FAAP100 antibody specific to Human FAAP100 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human FAAP100 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human FAAP100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human FAAP100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验This protocol is based on enrichment from 1 x 107 leucocytes (MNC). It is scalable from 1 x 107 –2 x 109 cells. Keep the cells and buffers cold (2–8°C) during the whole process. 1) Transfer 100 μl (1 x 107 ) leucocytes in Buffer
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人S100 蛋白 ( S100 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 S100 单抗包被于酶标板上,标准品和样品中的 S100与单抗结合,加入生物素化的抗人 S100
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