Human Serine/threonine- protein kinase NLK, NLK ELISA KIT
Product Name:Human Serine/threonine- protein kinase NLK, NLK ELISA KIT Packing:96T
Catalog No.:ELI-44037h
Gene Name:NLK
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Serine(threonine-protein kinase NLK(NLK
Alternative Name:NLK,Serine(threonine-protein kinase NLK(NLK Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Serine/threonine- protein kinase NLK, NLK ELISA KIT allows for the in vitro quantitative determination of Serine(threonine-protein kinase NLK(NLK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Serine/threonine- protein kinase NLK, NLK ELISA KIT has been pre-coated with an Serine(threonine-protein kinase NLK(NLK antibody specific to Serine(threonine-protein kinase NLK(NLK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Serine(threonine-protein kinase NLK(NLK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine(threonine-protein kinase NLK(NLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Serine(threonine-protein kinase NLK(NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!