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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Inositol polyphosphate 5-phosphatase K,INPP5K
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Inositol polyphosphate 5- phosphatase K, INPP5K ELISA KIT
Product Name:Human Inositol polyphosphate 5- phosphatase K, INPP5K ELISA KIT
Packing:96T
Catalog No.:ELI-43735h
Gene Name:INPP5K
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Inositol polyphosphate 5-phosphatase K(INPP5K
Alternative Name:INPP5K,Inositol polyphosphate 5-phosphatase K(INPP5K
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Inositol polyphosphate 5- phosphatase K, INPP5K ELISA KIT allows for the in vitro quantitative determination of Inositol polyphosphate 5-phosphatase K(INPP5K concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Inositol polyphosphate 5- phosphatase K, INPP5K ELISA KIT has been pre-coated with an Inositol polyphosphate 5-phosphatase K(INPP5K antibody specific to Inositol polyphosphate 5-phosphatase K(INPP5K .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Inositol polyphosphate 5-phosphatase K(INPP5K and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inositol polyphosphate 5-phosphatase K(INPP5K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Inositol polyphosphate 5-phosphatase K(INPP5K in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
., Lapetina, E.G., and Niedel, J.E. 1985. Pertussis toxin inhibits chemotactic peptide‐stimulated generation of inositol phosphates and lysosomal enzyme secretion in human leukemic (HL‐60) cells. Proc. Natl. Acad. Sci. U.S.A. 82:3277‐3280
【分享】Human press METHODS IN MOLECULAR BIOLOGY
Culture. Edited by Walker, John M.1984 6. Plant Cell and Tissue Culture. Edited by Walker, John M., 1984 7. Gene Transfer and Expression Protocols. Edited by Murray, Edward J., 1992 8. Practical Molecular Virology. Edited by Collins, Mary K., 1992
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