- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-43473h
- 2025年06月26日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Glutaryl-CoA dehydrogenase,mitochondrial,GCDH
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Glutaryl-CoA dehydrogenase mitochondrial, GCDH ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GCDH,GCD; ACAD5; glutaryl-Coenzyme A dehydrogenase; glutaryl-CoA dehydrogenase,Glutaryl-CoA dehydrogenase mitochondrial
Search name
Human GCDH ELISA KIT ,Human GCD ELISA KIT ,Human ACAD5 ELISA KIT ,Human glutaryl-Coenzyme A dehydrogenase ELISA KIT ,Human glutaryl-CoA dehydrogenase ELISA KIT ,Human Glutaryl-CoA dehydrogenase mitochondrial ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human GCDH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Human Glutaryl-CoA dehydrogenase mitochondrial, GCDH ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCDH, During the reaction,GCDHin the sample or standard competes with a fixed amount of biotin-labeled GCDH for sites on a pre-coated Monoclonal antibody specific to GCDH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GCDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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against the human pyruvate dehydrogenase (PDH) complex (Table 12.6), a large, multienzyme assembly residing in the mitochondrial matrix and consisting of three catalytic activities: pyruvate dehydrogenase, dihydrolipoyl transacetylase and dihydrolipoyl dehydrogenase
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