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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Replication protein A 30 kDa subunit,RPA4
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Replication protein A 30 kDa subunit, RPA4 ELISA KIT
Product Name:Human Replication protein A 30 kDa subunit, RPA4 ELISA KIT
Packing:96T
Catalog No.:ELI-42847h
Gene Name:RPA4
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Replication protein A 30 kDa subunit(RPA4
Alternative Name:RPA4,Replication protein A 30 kDa subunit(RPA4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Replication protein A 30 kDa subunit, RPA4 ELISA KIT allows for the in vitro quantitative determination of Replication protein A 30 kDa subunit(RPA4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Replication protein A 30 kDa subunit, RPA4 ELISA KIT has been pre-coated with an Replication protein A 30 kDa subunit(RPA4 antibody specific to Replication protein A 30 kDa subunit(RPA4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Replication protein A 30 kDa subunit(RPA4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Replication protein A 30 kDa subunit(RPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Replication protein A 30 kDa subunit(RPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
of phosphorylation sites in proteins: Construction of a phosphorylatable human interferon alpha. Proc. Natl. Acad. Sci. U.S.A. 86:558‐562. Loizos, N. and Darst, S.A. 1998. Mapping protein
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
interactions. Nat. Biotechnol. 18:847‐851. Ramani, A.K., Bunescu, R.C., Mooney, R.J., and Marcotte, E.M. 2005. Consolidating the set of known human protein‐protein interactions
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