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- 详细信息
- 文献和实验
- 技术资料
- 库存:
25
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Protein LSM14 homolog B,LSM14B
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Protein LSM14 homolog B,LSM14B ELISA KIT
Product Name:Human Protein LSM14 homolog B,LSM14B ELISA KIT
Packing:96T
Catalog No.:Human LSM14B ELISA KIT
Gene Name:LSM14B
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human LSM14B
Alternative Name:Human LSM14B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human LSM14B ELISA KIT allows for the in vitro quantitative determination of Human LSM14B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LSM14B ELISA KIT has been pre-coated with an Human LSM14B antibody specific to Human LSM14B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human LSM14B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human LSM14B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human LSM14B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
. The fluorescent toolbox for assessing protein location and function. Science 312:217‐224. Gordon, G.W., Berry, G., Liang, X.H., Levine, B., and Herman, B. 1998. Quantitative
Co-Expression of human DNA primase p49-p58 subunits
Adapted from W.C.Copeland,Protein Exp.& Purif 9,(1997)1-9 and Schneider,et.al.,JBC 273:34 (1998)21608-21615. Obtained from Fanning lab and revised by LSM,7/23/01 Obtained cell stock of BL21(DE3)cells cotransformed with pET21a-Hp49 (ampicillin
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