- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-41236h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ribonuclease P protein subunit p14,RPP14
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Ribonuclease P protein subunit p14, RPP14 ELISA KIT
Product Name:Human Ribonuclease P protein subunit p14, RPP14 ELISA KIT
Packing:96T
Catalog No.:ELI-41236h
Gene Name:RPP14
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Human Ribonuclease P protein subunit p14
Alternative Name:RPP14,Human Ribonuclease P protein subunit p14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ribonuclease P protein subunit p14, RPP14 ELISA KIT allows for the in vitro quantitative determination ofHuman Ribonuclease P protein subunit p14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ribonuclease P protein subunit p14, RPP14 ELISA KIT has been pre-coated with an RPP14 antibody specific to Human Ribonuclease P protein subunit p14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Ribonuclease P protein subunit p14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ribonuclease P protein subunit p14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human Ribonuclease P protein subunit p14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Ribonuclease P (RNase P) from Escherichia coli is a transfer RNA (tRNA)-processing enzyme and consists of a catalytic RNA subunit (M1 RNA) and a protein component (C5 protein). M1GS, a gene-targeting ribozyme derived from M1 RNA, can cleave
Ribonuclease A (RNase A), DNase-free
pancreas.Molecular Weight13.7 kDa monomer.ApplicationsPlasmid and genomic DNA preparation (3, 4), ..Removal of RNA from recombinant protein preparations.Ribonuclease protection assays (3).Mapping single-base mutations in DNA or RNA (5, 6).Quality Control
P is the catalytic subunit of the enzyme. Cell. 1983, 35 (3 Pt 2): 849-57. PMID 6197186. ^ (英文)1989年度诺贝尔化学奖 ^ 3.0 3.1 (英文)Jarrous N, Reiner R. Human RNase P: a tRNA-processing enzyme and transcription factor. Nucleic Acids Res.. 2007, 35
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