- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-40918h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ras-related GTP-binding protein D,RRAGD
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ras- related GTP- binding protein D, RRAGD ELISA KIT
Product Name:Human Ras- related GTP- binding protein D, RRAGD ELISA KIT
Packing:96T
Catalog No.:ELI-40918h
Gene Name:RRAGD
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Ras-related GTP-binding protein D(RRAGD
Alternative Name:RRAGD,Ras-related GTP-binding protein D(RRAGD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ras- related GTP- binding protein D, RRAGD ELISA KIT allows for the in vitro quantitative determination of Ras-related GTP-binding protein D(RRAGD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ras- related GTP- binding protein D, RRAGD ELISA KIT has been pre-coated with an Ras-related GTP-binding protein D(RRAGD antibody specific to Ras-related GTP-binding protein D(RRAGD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ras-related GTP-binding protein D(RRAGD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ras-related GTP-binding protein D(RRAGD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ras-related GTP-binding protein D(RRAGD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
. A physicochemical study f protein G, a molecule with unique immunoglobulin G-binding properties. J. Biol. Chem. 261:10240-10247. Harlow, E. and Lane, D. 1988. Antibodies. A laboratory manual. Cold Spring Harbor Laboratory. Kronvall, G., Seal, U. S
are not as important, as you are looking for a relatively small number of differences and so can devote more time to the identification of each protein.2D electrophoresis is performed in two steps(1) Isoelectric focusing (IEF) (First dimension electrophoresis): is used
protein interactions may be at the heart of aging-related diseases. Finally, quality control issues related to implementation of high throughput technologies are addressed, to underscore the importance of minimizing bias and randomizing human and technical
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