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- 详细信息
- 文献和实验
- 技术资料
- 库存:
25
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Small ubiquitin-related modifier 4,SUMO4
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Small ubiquitin-related modifier 4,SUMO4 ELISA KIT
Product Name:Human Small ubiquitin-related modifier 4,SUMO4 ELISA KIT
Packing:96T
Catalog No.:Human SUMO4 ELISA KIT
Gene Name:SUMO4
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human SUMO4
Alternative Name:Human SUMO4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SUMO4 ELISA KIT allows for the in vitro quantitative determination of Human SUMO4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SUMO4 ELISA KIT has been pre-coated with an Human SUMO4 antibody specific to Human SUMO4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human SUMO4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human SUMO4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human SUMO4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
both in vitro and in cells. Because another protein modifier, NEDD8, itself regulates aspects of the ubiquitin system, basic protocols on neddylation are also included in this unit. Keywords: Ubiquitin; ubiquitylation; proteasomal degradation; protein
Small ubiquitin-like modifier (SUMO) 蛋白的修饰及结合於调控细胞及病毒功能之研究:SUMO是近年来被发现的数种与ubiquitin相类似的蛋白质中的一种。它可经由类似ubiquitination的过程而与目标蛋白质上特定的lysine的支链形成共价键。而修饰目标蛋白质,这个过程称为SUMOylation。 多种基因转录因子,讯息传递分子及病毒蛋白已被证实会被SUMO修饰。与ubiquitination不同的是,sumoylation不会促进其目标
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
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