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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Protein unc-50 homolog,UNC50
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Protein unc- 50 homolog, UNC50 ELISA KIT
Product Name:Human Protein unc- 50 homolog, UNC50 ELISA KIT
Packing:96T
Catalog No.:ELI-40636h
Gene Name:UNC50
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Protein unc-50 homolog(UNC50
Alternative Name:UNC50,Protein unc-50 homolog(UNC50
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein unc- 50 homolog, UNC50 ELISA KIT allows for the in vitro quantitative determination of Protein unc-50 homolog(UNC50 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein unc- 50 homolog, UNC50 ELISA KIT has been pre-coated with an Protein unc-50 homolog(UNC50 antibody specific to Protein unc-50 homolog(UNC50 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein unc-50 homolog(UNC50 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein unc-50 homolog(UNC50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein unc-50 homolog(UNC50 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验samples, MPC-15 for 1–15 ml samples and MPC-50 for 15–50 ml samples. Mixer allowing both tilting and rotation. Buffer 1: PBS (without Ca2 and Mg2 ) w/0.1% BSA and 2 mM EDTA, pH 7.4. Buffer 2: PBS pH 7.4 (without Ca2 and Mg2 ).
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
相关专题 在蛋白质定量试验中,最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒(BCA Protein Assay Kit)。因为BCA法蛋白定量具有很多优点,如1.实验操作十分简单方便;2.实验耗时短,快速;3.试剂灵敏度高,实验结果准确;4.所用的试剂稳定性好,而且经济实用,抗干扰能力强等。 实验原理: BCA(bicinchoninic acid)法蛋白浓度定量
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