- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-40418h
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin carboxyl-terminal hydrolase 20,USP20
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ubiquitin carboxyl- terminal hydrolase 20, USP20 ELISA KIT
Product Name:Human Ubiquitin carboxyl- terminal hydrolase 20, USP20 ELISA KIT
Packing:96T
Catalog No.:ELI-40418h
Gene Name:USP20
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ubiquitin carboxyl-terminal hydrolase 20(USP20
Alternative Name:USP20,Ubiquitin carboxyl-terminal hydrolase 20(USP20
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin carboxyl- terminal hydrolase 20, USP20 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin carboxyl-terminal hydrolase 20(USP20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin carboxyl- terminal hydrolase 20, USP20 ELISA KIT has been pre-coated with an Ubiquitin carboxyl-terminal hydrolase 20(USP20 antibody specific to Ubiquitin carboxyl-terminal hydrolase 20(USP20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase 20(USP20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase 20(USP20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin carboxyl-terminal hydrolase 20(USP20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
【分享】Human press METHODS IN MOLECULAR BIOLOGY
.Ubiquitin-Proteasome Protocols Edited by Patterson, Cam 2005 302.Handbook of ELISPOT Methods and Protocols Edited by Kalyuzhny, Alexander E. 2005 303.NanoBiotechnology Protocols Edited by Rosenthal, Sandra J 2005 304.Human Retrovirus Protocols Virology
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