- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-40320h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Zinc finger E-box-binding homeobox 1,ZEB1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Zinc finger E- box- binding homeobox 1, ZEB1 ELISA KIT
Product Name:Human Zinc finger E- box- binding homeobox 1, ZEB1 ELISA KIT
Packing:96T
Catalog No.:ELI-40320h
Gene Name:ZEB1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Zinc finger E-box-binding homeobox 1(ZEB1
Alternative Name:ZEB1,Zinc finger E-box-binding homeobox 1(ZEB1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Zinc finger E- box- binding homeobox 1, ZEB1 ELISA KIT allows for the in vitro quantitative determination of Zinc finger E-box-binding homeobox 1(ZEB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Zinc finger E- box- binding homeobox 1, ZEB1 ELISA KIT has been pre-coated with an Zinc finger E-box-binding homeobox 1(ZEB1 antibody specific to Zinc finger E-box-binding homeobox 1(ZEB1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Zinc finger E-box-binding homeobox 1(ZEB1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zinc finger E-box-binding homeobox 1(ZEB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Zinc finger E-box-binding homeobox 1(ZEB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
editing using sequence‐specific nucleases. Sequence‐specific nucleases are composed of a domain that binds to the target gene via zinc‐finger or TAL effector‐based DNA recognition motifs. The DNA binding part is fused to the nonspecific nuclease domain
of three-finger chimeric ZFPs. (i) ZFPs composed exclusively of the human zinc fingers described above. We selected eighteen zinc-finger domains after considering each domain's DNA-binding specificity and affinity (see Supplementary Table 1 online). (ii) ZFPs
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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