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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Vesicular glutamate transporter 1,SLC17A7
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Vesicular glutamate transporter 1, SLC17A7 ELISA KIT
Product Name:Human Vesicular glutamate transporter 1, SLC17A7 ELISA KIT
Packing:96T
Catalog No.:ELI-39850h
Gene Name:SLC17A7
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Vesicular glutamate transporter 1(SLC17A7
Alternative Name:SLC17A7,Vesicular glutamate transporter 1(SLC17A7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Vesicular glutamate transporter 1, SLC17A7 ELISA KIT allows for the in vitro quantitative determination of Vesicular glutamate transporter 1(SLC17A7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Vesicular glutamate transporter 1, SLC17A7 ELISA KIT has been pre-coated with an Vesicular glutamate transporter 1(SLC17A7 antibody specific to Vesicular glutamate transporter 1(SLC17A7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Vesicular glutamate transporter 1(SLC17A7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Vesicular glutamate transporter 1(SLC17A7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Vesicular glutamate transporter 1(SLC17A7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验A Homogeneous Assay to Assess GABA Transporter Activity
.A., and Scholze, P. 2002. Bi‐directional transport of GABA in human embryonic kidney (HEK) cells stably expressing the rat GABA transporter GAT‐1. Br. J. Pharmacol. 135:93‐102. Voytas
for the functional production of the rat vesicular glutamate transporter (VGLUT2) and the human glucose transporter (GLUT1) (5).
cDNA Expression Cloning in Mammalian Cells
Erickson, J.D., Eiden, L.E., and Hoffman, B.J. 1992. Expression cloning of a reserpine‐sensitive vesicular monoamine transporter. Proc. Natl. Acad. Sci. U.S.A. 89:10,993‐10,997.
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