- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-38892h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human E3 ubiquitin-protein ligase RING2,RNF2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human E3 ubiquitin- protein ligase RING2, RNF2 ELISA KIT
Product Name:Human E3 ubiquitin- protein ligase RING2, RNF2 ELISA KIT
Packing:96T
Catalog No.:ELI-38892h
Gene Name:RNF2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:E3 ubiquitin-protein ligase RING2(RNF2
Alternative Name:RNF2,E3 ubiquitin-protein ligase RING2(RNF2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human E3 ubiquitin- protein ligase RING2, RNF2 ELISA KIT allows for the in vitro quantitative determination of E3 ubiquitin-protein ligase RING2(RNF2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human E3 ubiquitin- protein ligase RING2, RNF2 ELISA KIT has been pre-coated with an E3 ubiquitin-protein ligase RING2(RNF2 antibody specific to E3 ubiquitin-protein ligase RING2(RNF2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E3 ubiquitin-protein ligase RING2(RNF2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin-protein ligase RING2(RNF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of E3 ubiquitin-protein ligase RING2(RNF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assays for RING Family Ubiquitin Ligases
classes of E3 ligases, the HECT domain and the RING finger-containing ligases. RING finger-based ubiquitination utilizes RING-containing protein subunits, or proteins with intrinsic RING domains, to catalyze the formation of polyubiquitin chains
Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
ubiquitin protein ligase activity to wild-type (but not mutant) Parkin proteins. Although the individual components of the proposed Parkin ubiquitin ligase complex in the normal human brain remain to be identified and the E3 ligase effect of Parkin function
Studies of the Ubiquitin Proteasome System
and Translation Systems In Vitro Production of Ubiquitin‐Protein Ligases (E3s) Basic Protocol 24: Expression of Ring‐Finger E3s in Bacterial Lysates Alternate Protocol 14: Expression of HECT and U‐Box E3s in Bacterial Lysates Alternate Protocol 15: Expression
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