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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Potassium voltage-gated channel subfamily B member 2,KCN
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Potassium voltage-gated channel subfamily B member 2, KCNB2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Potassium voltage-gated channel subfamily B member 2,Voltage-gated potassium channel subunit Kv2.2, KCNB2,KV2.2,delayed rectifier potassium channel protein,potassium channel Kv2.2,potassium voltage-gated channel subfamily B member 2,voltage-gated potassium channel subunit Kv2.2,potassium voltage-gated channel, Shab-related subfamily member 2
Search name
Human Potassium voltage-gated channel subfamily B member 2 ELISA KIT ,Human Voltage-gated potassium channel subunit Kv2.2 ELISA KIT ,Human KCNB2 ELISA KIT ,Human KV2.2 ELISA KIT ,Human delayed rectifier potassium channel protein ELISA KIT ,Human potassium channel Kv2.2 ELISA KIT ,Human potassium voltage-gated channel subfamily B member 2 ELISA KIT ,Human voltage-gated potassium channel subunit Kv2.2 ELISA KIT ,Human potassium voltage-gated channel ELISA KIT ,Human Shab-related subfamily member 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human potassium voltage-gated channel subfamily b member 2,KCNB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to KCNB2, During the reaction, KCNB2 in the sample or standard competes with a fixed amount of biotin-labeled KCNB2 for sites on a pre-coated Monoclonal antibody specific to KCNB2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KCNB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of Potassium Channel Binding
Figure 1.17.1 Displacement of [3 H]P 1075 whole‐cell binding to KATP channels in rat A10 cells using P 1075 and two potassium channel–opener compounds. The data was fitted using GraphPad Prism program. Figure kindly contributed by R. Davis Taber
The Loose Patch Voltage Clamp Technique
until it was revived by St�hmer and Almers (1982). Almers, St�hmer, and their collaborators used the loose patch-clamp to study a wide range of muscle sodium and potassium channel properties, including mobility, inactivation, and spatial distribution (St�hmer
Characterization of Calcium Channel Binding
.P., Fox, M., Rees, M. and Perez‐Reyes, E. 1998. Cloning and characterization of alpha1H from human heart, a member of the T‐type Ca2+ channel gene family. Circ. Res. 83:103‐109.
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