Human Probable G- protein coupled receptor 45, GPR45 ELISA KIT
Product Name:Human Probable G- protein coupled receptor 45, GPR45 ELISA KIT
Packing:96T
Catalog No.:ELI-39004h
Gene Name:GPR45
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Probable G-protein coupled receptor 45(GPR45
Alternative Name:GPR45,Probable G-protein coupled receptor 45(GPR45
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Probable G- protein coupled receptor 45, GPR45 ELISA KIT allows for the in vitro quantitative determination of Probable G-protein coupled receptor 45(GPR45 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Probable G- protein coupled receptor 45, GPR45 ELISA KIT has been pre-coated with an Probable G-protein coupled receptor 45(GPR45 antibody specific to Probable G-protein coupled receptor 45(GPR45 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Probable G-protein coupled receptor 45(GPR45 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Probable G-protein coupled receptor 45(GPR45, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Probable G-protein coupled receptor 45(GPR45 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!