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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human A/G-specific adenine DNA glycosylase,MUTYH
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human A/G- specific adenine DNA glycosylase, MUTYH ELISA KIT
Product Name:Human A/G- specific adenine DNA glycosylase, MUTYH ELISA KIT
Packing:96T
Catalog No.:ELI-38607h
Gene Name:MUTYH
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:A(G-specific adenine DNA glycosylase(MUTYH
Alternative Name:MUTYH,A(G-specific adenine DNA glycosylase(MUTYH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human A/G- specific adenine DNA glycosylase, MUTYH ELISA KIT allows for the in vitro quantitative determination of A(G-specific adenine DNA glycosylase(MUTYH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human A/G- specific adenine DNA glycosylase, MUTYH ELISA KIT has been pre-coated with an A(G-specific adenine DNA glycosylase(MUTYH antibody specific to A(G-specific adenine DNA glycosylase(MUTYH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for A(G-specific adenine DNA glycosylase(MUTYH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain A(G-specific adenine DNA glycosylase(MUTYH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of A(G-specific adenine DNA glycosylase(MUTYH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
. Horton, J.R., Liebert, K., Hattman, S., Jeltsch, A., and Cheng, X. 2005. Transition from nonspecific to specific DNA interactions along the substrate‐recognition pathway of dam methyltransferase. Cell 121:349
in such cell death. By introducing human cDNA encoding a nuclear form of 8-oxoG DNA glycosylase (hOGG1-1a) into immortalized mouse embryo fibroblasts lacking Ogg1 gene, we established a cell line which selectively accumulates 8-oxoguanine in mitochondrial DNA
) Sequencher(Mac and Windows,RCR has a site license) Gene Construction Kit(Mac only) Vector NTI(Mac and Windows) Plasmid Premier (Windows only) DNA Strider is simple,but still elegant Vector NTI Vector NTI puts the plasmid map at the center
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