- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-38096h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Purine nucleoside phosphorylase,PNP
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Purine nucleoside phosphorylase, PNP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PNP,NP,Pnp1,nucleoside phosphorylase,purine nucleoside phosphorylase; PRO1837; PUNP; HEL-S-156an; epididymis secretory sperm binding protein Li 156an; inosine phosphorylase; inosine-guanosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase
Search name
Human PNP ELISA KIT ,Human NP ELISA KIT ,Human Pnp1 ELISA KIT ,Human nucleoside phosphorylase ELISA KIT ,Human purine nucleoside phosphorylase ELISA KIT ,Human PRO1837 ELISA KIT ,Human PUNP ELISA KIT ,Human HEL-S-156an ELISA KIT ,Human epididymis secretory sperm binding protein Li 156an ELISA KIT ,Human inosine phosphorylase ELISA KIT ,Human inosine-guanosine phosphorylase ELISA KIT ,Human purine-nucleoside:orthophosphate ribosyltransferase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Purine nucleoside phosphorylase, PNP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNP, During the reaction, PNP in the sample or standard competes with a fixed amount of biotin-labeled PNP for sites on a pre-coated Monoclonal antibody specific to PNP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Tumor Sensitization to Purine Analogs by E. coli PNP
This chapter describes an approach to destroying malignant cells by effectively changing the tumor phenotype through the delivery of Escherichia coli purine nucleo-side phosphorylase (PNP). In the presence of nucleoside prodrugs
Production and Release Testing of Ovine Atadenovirus Vectors
purine nucleoside phosphorylase (PNP) have been developed for first time use in humans in a phase I trial for the treatment of prostate cancer. Here we describe methods for their production together with several quality-control assays. In particular
佚名 嘌呤核苷酸可以在核苷酸酶的催化下，脱去磷酸成为嘌呤核苷，嘌呤核苷在嘌呤核苷磷酸化酶(purine nucleoside phosphorylase,PNP)的催化下转变为嘌呤。嘌呤核苷及嘌呤又可经水解，脱氨及氧化作用生成尿酸(图8－15) 图8－15 嘌呤核苷酸的分解代谢 哺乳动物中，腺苷和脱氧腺苷不能由PNP分解，而是在核苷
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