- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-38640h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Hypoxanthine-guanine phosphoribosyltransferase,HPRT1 ELI
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human Hypoxanthine- guanine phosphoribosyltransferase, HPRT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HPRT1,HGPRT; HPRT; HGPRTase; hypoxanthine-guanine phosphoribosyltransferase 1; hypoxanthine phosphoribosyltransferase 1, Hypoxanthine-guanine phosphoribosyltransferase
Search name
Human HPRT1 ELISA KIT ,Human HGPRT ELISA KIT ,Human HPRT ELISA KIT ,Human HGPRTase ELISA KIT ,Human hypoxanthine-guanine phosphoribosyltransferase 1 ELISA KIT ,Human hypoxanthine phosphoribosyltransferase 1 ELISA KIT ,Human Hypoxanthine-guanine phosphoribosyltransferase ELISA KIT
Intended use
Human HPRT1 ELISA KIT allows for the in vitro quantitative determination of human hypoxanthine-guanine phosphoribosyltransferase,HGPRT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human HPRT1 ELISA KITt has been pre-coated with an antibody specific to HGPRT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HGPRT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HGPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HGPRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验The T-cell cloning assay, which detects mutations in the gene for hypoxanthine-guanine phosphoribosyltransferase (HPRT ), is the most well-developed reporter system for studying specific locus mutation in human somatic cells. The assay
Molecular Analysis of Mutations in the Human HPRT Gene
The HPRT assay uses incorporation of toxic nucleotide analogs to select for cells lacking the purine scavenger enzyme hypoxanthine-guanine phosporibosyltransferase. A major advantage of this assay is the ability to isolate mutant cells
Human Xanthine Oxidoreductase Determination by a Competitive ELISA
Xanthine oxidoreductase (XO) catalyses the oxidation of hypoxanthine to xanthine and of the latter to uric acid. The enzyme is present in traces in most of human tissues, including plasma, being more abundant in milk, liver, and intestine (1).
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