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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Basic salivary proline-rich protein 1,PRB1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Basic salivary proline- rich protein 1, PRB1 ELISA KIT
Product Name:Human Basic salivary proline- rich protein 1, PRB1 ELISA KIT
Packing:96T
Catalog No.:ELI-37852h
Gene Name:PRB1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Basic salivary proline-rich protein 1(PRB1
Alternative Name:PRB1,Basic salivary proline-rich protein 1(PRB1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Basic salivary proline- rich protein 1, PRB1 ELISA KIT allows for the in vitro quantitative determination of Basic salivary proline-rich protein 1(PRB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Basic salivary proline- rich protein 1, PRB1 ELISA KIT has been pre-coated with an Basic salivary proline-rich protein 1(PRB1 antibody specific to Basic salivary proline-rich protein 1(PRB1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Basic salivary proline-rich protein 1(PRB1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Basic salivary proline-rich protein 1(PRB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Basic salivary proline-rich protein 1(PRB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
. Kawas, S.A., Rahim, Z.H., and Ferguson, D.B. 2012. Potential uses of human salivary protein and peptide analysis in the diagnosis of disease. Arch. Oral Biol. 57:1‐9.
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
interactions. Nat. Biotechnol. 18:847‐851. Ramani, A.K., Bunescu, R.C., Mooney, R.J., and Marcotte, E.M. 2005. Consolidating the set of known human protein‐protein interactions
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