Target Protein Name:Lipid phosphate phosphohydrolase 1(PPAP2A
Alternative Name:PPAP2A,Lipid phosphate phosphohydrolase 1(PPAP2A Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Lipid phosphate phosphohydrolase 1, PPAP2A ELISA KIT allows for the in vitro quantitative determination of Lipid phosphate phosphohydrolase 1(PPAP2A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Lipid phosphate phosphohydrolase 1, PPAP2A ELISA KIT has been pre-coated with an Lipid phosphate phosphohydrolase 1(PPAP2A antibody specific to Lipid phosphate phosphohydrolase 1(PPAP2A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lipid phosphate phosphohydrolase 1(PPAP2A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lipid phosphate phosphohydrolase 1(PPAP2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Lipid phosphate phosphohydrolase 1(PPAP2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!