- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 国产(香港)
- ELI-37279h
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Pituitary-specific positive transcription factor 1,POU1F
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human Pituitary- specific positive transcription factor 1, POU1F1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
POU1F1,CPHD1; GHF-1; PIT1; POU1F1a; Pit-1; POU domain, class 1, transcription factor 1; growth hormone factor 1; pituitary-specific positive transcription factor 1; POU class 1 homeobox 1
Search name
Human POU1F1 ELISA KIT ,Human CPHD1 ELISA KIT ,Human GHF-1 ELISA KIT ,Human PIT1 ELISA KIT ,Human POU1F1a ELISA KIT ,Human Pit-1 ELISA KIT ,Human POU domain ELISA KIT ,Human class 1 ELISA KIT ,Human transcription factor 1 ELISA KIT ,Human growth hormone factor 1 ELISA KIT ,Human pituitary-specific positive transcription factor 1 ELISA KIT ,Human POU class 1 homeobox 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Pituitary- specific positive transcription factor 1, POU1F1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pituitary-specific positive transcription factor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pituitary-specific positive transcription factor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pituitary-specific positive transcription factor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pituitary-specific positive transcription factor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验Assays for Transcription Factor Activity
Most transcription activator proteins have three important features that can be probed at the molecular level: They bind to specific sequences near promoters, they can be interconverted between active and inactive forms by covalent or noncovalent
from chromatin immunoprecipitation (ChIP) experiments, and therefore revealing allele-specific gene expression and transcription factor binding, respectively. The throughput of allelic imbalance assays ranges from single variant to the genome scale
Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Continuous nucleocytoplasmic shuttling of signal transducer and activator of transcription (STAT) proteins is a key to understand their function as cytokine-responsive transcription factors. STATs enter the nucleus both by carrier-dependent
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