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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Proteasome subunit alpha type-5,PSMA5
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human Proteasome subunit alpha type- 5, PSMA5 ELISA KIT
Product Name:Human Proteasome subunit alpha type- 5, PSMA5 ELISA KIT
Packing:96T
Catalog No.:ELI-36896h
Gene Name:PSMA5
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Proteasome subunit alpha type-5(PSMA5
Alternative Name:PSMA5,Proteasome subunit alpha type-5(PSMA5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Proteasome subunit alpha type- 5, PSMA5 ELISA KIT allows for the in vitro quantitative determination of Proteasome subunit alpha type-5(PSMA5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Proteasome subunit alpha type- 5, PSMA5 ELISA KIT has been pre-coated with an Proteasome subunit alpha type-5(PSMA5 antibody specific to Proteasome subunit alpha type-5(PSMA5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Proteasome subunit alpha type-5(PSMA5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proteasome subunit alpha type-5(PSMA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Proteasome subunit alpha type-5(PSMA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
Control Interferon-alpha treated
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