Human Myosin-binding protein C

Human Myosin- binding protein C cardiac- type, MYBPC3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MYBPC3,CMD1MM; CMH4; FHC; LVNC10; MYBP-C; C-protein, cardiac muscle isoform; myosin-binding protein C, cardiac-type; myosin binding protein C cardiac

Search name

Human MYBPC3 ELISA KIT ,Human CMD1MM ELISA KIT ,Human CMH4 ELISA KIT ,Human FHC ELISA KIT ,Human LVNC10 ELISA KIT ,Human MYBP-C ELISA KIT ,Human C-protein ELISA KIT ,Human cardiac muscle isoform ELISA KIT ,Human myosin-binding protein C ELISA KIT ,Human  cardiac-type ELISA KIT ,Human myosin binding protein C cardiac ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human myosin-binding protein c, cardiac-type,Cardiac MyBP-C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiac MyBP-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cardiac MyBP-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cardiac MyBP-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cardiac MyBP-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual download