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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Sodium/myo-inositol cotransporter,SLC5A3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human Sodium/myo- inositol cotransporter, SLC5A3 ELISA KIT
Product Name:Human Sodium/myo- inositol cotransporter, SLC5A3 ELISA KIT
Packing:96T
Catalog No.:ELI-35920h
Gene Name:SLC5A3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Sodium(myo-inositol cotransporter(SLC5A3
Alternative Name:SLC5A3,Sodium(myo-inositol cotransporter(SLC5A3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Sodium/myo- inositol cotransporter, SLC5A3 ELISA KIT allows for the in vitro quantitative determination of Sodium(myo-inositol cotransporter(SLC5A3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Sodium/myo- inositol cotransporter, SLC5A3 ELISA KIT has been pre-coated with an Sodium(myo-inositol cotransporter(SLC5A3 antibody specific to Sodium(myo-inositol cotransporter(SLC5A3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sodium(myo-inositol cotransporter(SLC5A3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sodium(myo-inositol cotransporter(SLC5A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Sodium(myo-inositol cotransporter(SLC5A3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
操作条件、人员一致 边缘效应 蒸发/酶标板叠放 孵育加封板膜或盖板;避免叠放 孵育温度不均 校准温育箱;避开通风口、窗边等环境波动区 洗板不彻底 确保洗板仪正常;不减少洗液量与次数 注意!!! ELISA试剂盒11月活动即将来袭~敬请期待~ 部分热卖产品推荐 产品货号 产品名称 检测范围 灵敏度 97109ES Human IL18 ELISA Kit 125
提供多种高品质补体相关试剂,助力广大科研工作者的深入探索与研究。 以下是菲恩生物 补体 相关指标产品推荐 ELISA 试剂盒 货号 产品名称 灵敏度 EH5309 Human Anti-C3b antibody ELISA Kit 0.938ng/ml EH5308 Human Anti-CFB antibody ELISA Kit 0.469ng/ml EH5255
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