Target Protein Name:Gamma-secretase subunit APH-1B(APH1B
Alternative Name:APH1B,Gamma-secretase subunit APH-1B(APH1B Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Gamma- secretase subunit APH- 1B, APH1B ELISA KIT allows for the in vitro quantitative determination of Gamma-secretase subunit APH-1B(APH1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Gamma- secretase subunit APH- 1B, APH1B ELISA KIT has been pre-coated with an Gamma-secretase subunit APH-1B(APH1B antibody specific to Gamma-secretase subunit APH-1B(APH1B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gamma-secretase subunit APH-1B(APH1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gamma-secretase subunit APH-1B(APH1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Gamma-secretase subunit APH-1B(APH1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!