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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Alpha- ketoglutarate- dependent dioxygenase alkB homolog 3, ALKBH3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ALKBH3,ABH3; DEPC-1; DEPC1; PCA1; alkylated DNA repair protein alkB homolog 3; alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; prostate cancer antigen 1; prostate cancer antigen-1; alkB, alkylation repair homolog 3 (E. coli)
Search name
Human ALKBH3 ELISA KIT ,Human ABH3 ELISA KIT ,Human DEPC-1 ELISA KIT ,Human DEPC1 ELISA KIT ,Human PCA1 ELISA KIT ,Human alkylated DNA repair protein alkB homolog 3 ELISA KIT ,Human alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 ELISA KIT ,Human prostate cancer antigen 1 ELISA KIT ,Human prostate cancer antigen-1 ELISA KIT ,Human alkB ELISA KIT ,Human alkylation repair homolog 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Alpha- ketoglutarate- dependent dioxygenase alkB homolog 3, ALKBH3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALKBH3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ALKBH3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ALKBH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ALKBH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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