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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Activating molecule in BECN1-regulated autophagy protein
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Activating molecule in BECN1- regulated autophagy protein 1, AMBRA1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Ambra1,DCAF3; WDR94; DDB1 and CUL4 associated factor 3; WD repeat domain 94; activating molecule in BECN1-regulated autophagy protein 1; activating molecule in beclin-1-regulated autophagy; autophagy/beclin-1 regulator 1
Search name
Human Ambra1 ELISA KIT ,Human DCAF3 ELISA KIT ,Human WDR94 ELISA KIT ,Human DDB1 and CUL4 associated factor 3 ELISA KIT ,Human WD repeat domain 94 ELISA KIT ,Human activating molecule in BECN1-regulated autophagy protein 1 ELISA KIT ,Human activating molecule in beclin-1-regulated autophagy ELISA KIT ,Human autophagy/beclin-1 regulator 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Activating molecule in BECN1- regulated autophagy protein 1, AMBRA1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMBRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMBRA1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMBRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMBRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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