Human BAG family molecular chaperone regulator 5, BAG5 ELISA KIT
Product Name:Human BAG family molecular chaperone regulator 5, BAG5 ELISA KIT
Packing:96T
Catalog No.:ELI-34260h
Gene Name:BAG5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:BAG family molecular chaperone regulator 5(BAG5
Alternative Name:BAG5,BAG family molecular chaperone regulator 5(BAG5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human BAG family molecular chaperone regulator 5, BAG5 ELISA KIT allows for the in vitro quantitative determination of BAG family molecular chaperone regulator 5(BAG5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human BAG family molecular chaperone regulator 5, BAG5 ELISA KIT has been pre-coated with an BAG family molecular chaperone regulator 5(BAG5 antibody specific to BAG family molecular chaperone regulator 5(BAG5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BAG family molecular chaperone regulator 5(BAG5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BAG family molecular chaperone regulator 5(BAG5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of BAG family molecular chaperone regulator 5(BAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!