Target Protein Name:Choline(ethanolamine kinase(CHKB
Alternative Name:CHKB,Choline(ethanolamine kinase(CHKB Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Choline/ethanolamine kinase, CHKB ELISA KIT allows for the in vitro quantitative determination of Choline(ethanolamine kinase(CHKB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Choline/ethanolamine kinase, CHKB ELISA KIT has been pre-coated with an Choline(ethanolamine kinase(CHKB antibody specific to Choline(ethanolamine kinase(CHKB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Choline(ethanolamine kinase(CHKB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Choline(ethanolamine kinase(CHKB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Choline(ethanolamine kinase(CHKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!