- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-33171h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human G2/mitotic-specific cyclin-B2,CCNB2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human G2/mitotic- specific cyclin- B2, CCNB2 ELISA KIT
Product Name:Human G2/mitotic- specific cyclin- B2, CCNB2 ELISA KIT
Packing:96T
Catalog No.:ELI-33171h
Gene Name:CCNB2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:G2(mitotic-specific cyclin-B2(CCNB2
Alternative Name:CCNB2,G2(mitotic-specific cyclin-B2(CCNB2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human G2/mitotic- specific cyclin- B2, CCNB2 ELISA KIT allows for the in vitro quantitative determination of G2(mitotic-specific cyclin-B2(CCNB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human G2/mitotic- specific cyclin- B2, CCNB2 ELISA KIT has been pre-coated with an G2(mitotic-specific cyclin-B2(CCNB2 antibody specific to G2(mitotic-specific cyclin-B2(CCNB2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G2(mitotic-specific cyclin-B2(CCNB2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G2(mitotic-specific cyclin-B2(CCNB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of G2(mitotic-specific cyclin-B2(CCNB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
and Protocols. Edited by Pascol Bailon, 2000 148.DNA-protein interactions, Principle and protocols, 2nd, Edited by Tom Moss, 2000 149.The ELISA guide book, Edited by John Crowther, 2000 150.Complement, Methods and protocols. Edited by B. Paul Morgan. 2000
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
. shilei5794749 呵呵，这个ELISA检测基因突变比较意思。刚刚看了几篇文章，跟楼主分享一下。 文章：Detection of four b-thalassemia point mutations in Iranians using a PCR-ELISA genotyping system. 检测图中四个位置的基因突变。这个检测方法的四要素：a，DIG labed PCR products b，5’biotinylated
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