- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-32602h
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Protein FAM182A,FAM182A
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Protein FAM182A, FAM182A ELISA KIT
Product Name:Human Protein FAM182A, FAM182A ELISA KIT
Packing:96T
Catalog No.:ELI-32602h
Gene Name:FAM182A
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Protein FAM182A(FAM182A
Alternative Name:FAM182A,Protein FAM182A(FAM182A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein FAM182A, FAM182A ELISA KIT allows for the in vitro quantitative determination of Protein FAM182A(FAM182A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein FAM182A, FAM182A ELISA KIT has been pre-coated with an Protein FAM182A(FAM182A antibody specific to Protein FAM182A(FAM182A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein FAM182A(FAM182A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein FAM182A(FAM182A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein FAM182A(FAM182A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding.Analytical Biochemistry 72: 248-254.1976.•Stoscheck,CM.Quantitation of Protein.Methods in Enzymology 182: 50-69 (1990).
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选，选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时，又用传统的WB方式来验证这个结果。经过反复验证，都得出了同样的结果， Cell Based
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