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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Death ligand signal enhancer,KIAA0141
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Death ligand signal enhancer, KIAA0141 ELISA KIT
Product Name:Human Death ligand signal enhancer, KIAA0141 ELISA KIT
Packing:96T
Catalog No.:ELI-32190h
Gene Name:KIAA0141
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Death ligand signal enhancer(KIAA0141
Alternative Name:KIAA0141,Death ligand signal enhancer(KIAA0141
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Death ligand signal enhancer, KIAA0141 ELISA KIT allows for the in vitro quantitative determination of Death ligand signal enhancer(KIAA0141 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Death ligand signal enhancer, KIAA0141 ELISA KIT has been pre-coated with an Death ligand signal enhancer(KIAA0141 antibody specific to Death ligand signal enhancer(KIAA0141 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Death ligand signal enhancer(KIAA0141 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Death ligand signal enhancer(KIAA0141, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Death ligand signal enhancer(KIAA0141 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
, Bret A., 1995 41.Signal Transduction Protocols, 1st. Edited by D. A. Kendall, 1995 42. ELISA Theory and Practice. Edited by Crowther, John R., 1995 43. In Vitro Toxicity Testing Protocols, Edited by O'Hare, Sheila, 1995 44. Agrobacterium
-2R (IL-2 Receptor) alpha subunit primarily increases the affinity of ligand binding and is not known to contain a signaling domain, whereas the beta and gamma subunits participate in both ligand binding and signal transduction (Ref.1). The IL-2R signaling
Dual- and Triple-Color Fluorospot
3, and Cy5. 3. Fluorescence Enhancer (Mabtech) is used to improve the fluorescent signal. 实验步骤 The protocol describes double staining for the detection of human IFN-γ and IL-2. PVDF plates are coated with mAbs to IFN-γ and IL
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