Human Interleukin- 27 receptor subunit alpha, IL27RA ELISA KIT
Product Name:Human Interleukin- 27 receptor subunit alpha, IL27RA ELISA KIT
Packing:96T
Catalog No.:ELI-31530h
Gene Name:IL27RA
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Interleukin-27 receptor subunit alpha(IL27RA
Alternative Name:IL27RA,Interleukin-27 receptor subunit alpha(IL27RA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Interleukin- 27 receptor subunit alpha, IL27RA ELISA KIT allows for the in vitro quantitative determination of Interleukin-27 receptor subunit alpha(IL27RA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Interleukin- 27 receptor subunit alpha, IL27RA ELISA KIT has been pre-coated with an Interleukin-27 receptor subunit alpha(IL27RA antibody specific to Interleukin-27 receptor subunit alpha(IL27RA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Interleukin-27 receptor subunit alpha(IL27RA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Interleukin-27 receptor subunit alpha(IL27RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Interleukin-27 receptor subunit alpha(IL27RA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!