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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Polypeptide N-acetylgalactosaminyltransferase 13,GALNT13
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Polypeptide N- acetylgalactosaminyltransferase 13, GALNT13 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Galnt13,GalNAc T13,GalNAc-T13; H_NH0187G20.1; WUGSC:H_NH0187G20.1; GalNAc transferase 13; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 13; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13 (GalNAc-T13); polypeptide GalNAc transferase 13; pp-GaNTase 13; protein-UDP acetylgalactosaminyltransferase 13; polypeptide N-acetylgalactosaminyltransferase 13
Search name
Human Galnt13 ELISA KIT ,Human GalNAc T13 ELISA KIT ,Human GalNAc-T13 ELISA KIT ,Human H_NH0187G20.1 ELISA KIT ,Human WUGSC:H_NH0187G20.1 ELISA KIT ,Human GalNAc transferase 13 ELISA KIT ,Human UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase 13 ELISA KIT ,Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13 ELISA KIT ,Human GalNAc-T13 ELISA KIT ,Human polypeptide GalNAc transferase 13 ELISA KIT ,Human pp-GaNTase 13 ELISA KIT ,Human protein-UDP acetylgalactosaminyltransferase 13 ELISA KIT ,Human polypeptide N-acetylgalactosaminyltransferase 13 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Polypeptide N- acetylgalactosaminyltransferase 13, GALNT13 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GALNT13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GALNT13 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GALNT13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GALNT13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验The aim of this article is to present a unique in vitro folding technique for glycosyltransferases to generate active proteins that can be used for X-ray crystallographic and bioconjugation protocols. Although a number of in vitro refolding
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13 Synthesis of Peptide Bioconjugates
Bioconjugates play an important role in several fields of biomolecular and biomedicinal sciences. Protein/polypeptide-based conjugates with covalently attached epitope peptides are considered as potential synthetic vaccine candidates
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