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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Melanoma-associated antigen 12,MAGEA12
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Melanoma- associated antigen 12, MAGEA12 ELISA KIT
Product Name:Human Melanoma- associated antigen 12, MAGEA12 ELISA KIT
Packing:96T
Catalog No.:ELI-27886h
Gene Name:MAGEA12
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Melanoma-associated antigen 12(MAGEA12
Alternative Name:MAGEA12,Melanoma-associated antigen 12(MAGEA12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Melanoma- associated antigen 12, MAGEA12 ELISA KIT allows for the in vitro quantitative determination of Melanoma-associated antigen 12(MAGEA12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Melanoma- associated antigen 12, MAGEA12 ELISA KIT has been pre-coated with an Melanoma-associated antigen 12(MAGEA12 antibody specific to Melanoma-associated antigen 12(MAGEA12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Melanoma-associated antigen 12(MAGEA12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Melanoma-associated antigen 12(MAGEA12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Melanoma-associated antigen 12(MAGEA12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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