Target Protein Name:G-protein coupled receptor 182(GPR182
Alternative Name:GPR182,G-protein coupled receptor 182(GPR182 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human G- protein coupled receptor 182, GPR182 ELISA KIT allows for the in vitro quantitative determination of G-protein coupled receptor 182(GPR182 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human G- protein coupled receptor 182, GPR182 ELISA KIT has been pre-coated with an G-protein coupled receptor 182(GPR182 antibody specific to G-protein coupled receptor 182(GPR182 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G-protein coupled receptor 182(GPR182 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G-protein coupled receptor 182(GPR182, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of G-protein coupled receptor 182(GPR182 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!