Target Protein Name:Hereditary hemochromatosis protein(HFE
Alternative Name:HFE,Hereditary hemochromatosis protein(HFE Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Hereditary hemochromatosis protein, HFE ELISA KIT allows for the in vitro quantitative determination of Hereditary hemochromatosis protein(HFE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Hereditary hemochromatosis protein, HFE ELISA KIT has been pre-coated with an Hereditary hemochromatosis protein(HFE antibody specific to Hereditary hemochromatosis protein(HFE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Hereditary hemochromatosis protein(HFE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hereditary hemochromatosis protein(HFE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Hereditary hemochromatosis protein(HFE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!