- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-27721h
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Histone H1.5,HIST1H1B
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Histone H1.5, HIST1H1B ELISA KIT
Product Name:Human Histone H1.5, HIST1H1B ELISA KIT
Packing:96T
Catalog No.:ELI-27721h
Gene Name:HIST1H1B
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Histone H1.5(HIST1H1B
Alternative Name:HIST1H1B,Histone H1.5(HIST1H1B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Histone H1.5, HIST1H1B ELISA KIT allows for the in vitro quantitative determination of Histone H1.5(HIST1H1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Histone H1.5, HIST1H1B ELISA KIT has been pre-coated with an Histone H1.5(HIST1H1B antibody specific to Histone H1.5(HIST1H1B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone H1.5(HIST1H1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H1.5(HIST1H1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone H1.5(HIST1H1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, A.D., Rossi, J.J. and Morris, K.V. (2005) The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells, RNA, 12(2): 256-262.
HDAC（Histone Deacetylase）组蛋白去乙酰化酶活性测定及药物筛选方案
显象剂作用后产生荧光团或发光团。最终的发光团可以用ELISA读板仪或酶标仪读取分析，荧光团则可以通过荧光读板仪或荧光计分析。试剂盒里也提供了Hela细胞核提取物（HeLa Nuclear Extract）作为阳性对照，以Trichostatin A作为阴性对照。此分析很适合用于高流通量筛选。 而HDACi（组蛋白去乙酰化酶抑制剂）药物筛选试剂盒 -HDAC Inhibitor Drug Screening Kit（K340-100）采用了与HDAC Fluorometric Assay Kit
-designed using the Cenix algorithm from Ambion. Designs are currently available for >98% of the human, mouse, and rat genes in the RefSeq database. See the Pre-designed siRNA Catalog Page for more information. In addition, Ambion offers Silencer Validated
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