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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Probable helicase with zinc finger domain,HELZ
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Probable helicase with zinc finger domain, HELZ ELISA KIT
Product Name:Human Probable helicase with zinc finger domain, HELZ ELISA KIT
Packing:96T
Catalog No.:ELI-27617h
Gene Name:HELZ
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Probable helicase with zinc finger domain(HELZ
Alternative Name:HELZ,Probable helicase with zinc finger domain(HELZ
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Probable helicase with zinc finger domain, HELZ ELISA KIT allows for the in vitro quantitative determination of Probable helicase with zinc finger domain(HELZ concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Probable helicase with zinc finger domain, HELZ ELISA KIT has been pre-coated with an Probable helicase with zinc finger domain(HELZ antibody specific to Probable helicase with zinc finger domain(HELZ .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Probable helicase with zinc finger domain(HELZ and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Probable helicase with zinc finger domain(HELZ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Probable helicase with zinc finger domain(HELZ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
editing using sequence‐specific nucleases. Sequence‐specific nucleases are composed of a domain that binds to the target gene via zinc‐finger or TAL effector‐based DNA recognition motifs. The DNA binding part is fused to the nonspecific nuclease domain
of three-finger chimeric ZFPs. (i) ZFPs composed exclusively of the human zinc fingers described above. We selected eighteen zinc-finger domains after considering each domain's DNA-binding specificity and affinity (see Supplementary Table 1 online). (ii) ZFPs
large combinatorial libraries of artificial transcription factors comprising three or six zinc-finger domains, and selected transcription factor�DNA interactions able to upregulate several genes in human cells. Selected transcription factors
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