- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-27153h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
G antigen 3,GAGE3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human G antigen 3, GAGE3 ELISA KIT
Product Name:Human G antigen 3, GAGE3 ELISA KIT
Packing:96T
Catalog No.:ELI-27153h
Gene Name:GAGE3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:G antigen 3(GAGE3
Alternative Name:GAGE3,G antigen 3(GAGE3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human G antigen 3, GAGE3 ELISA KIT allows for the in vitro quantitative determination of G antigen 3(GAGE3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human G antigen 3, GAGE3 ELISA KIT has been pre-coated with an G antigen 3(GAGE3 antibody specific to G antigen 3(GAGE3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G antigen 3(GAGE3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G antigen 3(GAGE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of G antigen 3(GAGE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
人白细胞抗原(human leukocyte antigen，HLA)
相容性抗原分为“主要组织相容性抗原系统”(major histocompatibility antigen system)和“次要组织相容性系统”(minor histocompatibility antigen system)。前者指体细胞表面抗原能引起受体淋巴细胞强应答，产生强烈排斥反应；后者指引起弱应答和弱排斥反应。主要组织相容性抗原是一组抗原，由一组连锁基因编码。这组基因称为主要组织相容性复合体基因(major histocompatibility complex gene，MHC
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