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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Putative ATP-dependent Clp protease proteolytic subunit,
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Putative ATP-dependent Clp protease proteolytic subunit mitochondrial, CLPP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 3.4.21.92,Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial),Endopeptidase Clp,CLPP,ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; PRLTS3; ATP-dependent protease ClpAP, proteolytic subunit, human; endopeptidase Clp; caseinolytic mitochondrial matrix peptidase proteolytic subunit
Search name
Human CLPP ELISA KIT ,Human ClpP caseinolytic protease ELISA KIT ,Human ATP-dependent ELISA KIT ,Human proteolytic subunit homolog ELISA KIT ,Human ClpP caseinolytic peptidase ELISA KIT ,Human ATP-dependent ELISA KIT ,Human proteolytic subunit homolog ELISA KIT ,Human ClpP caseinolytic peptidase ATP-dependent ELISA KIT ,Human proteolytic subunit ELISA KIT ,Human PRLTS3 ELISA KIT ,Human ATP-dependent protease ClpAP ELISA KIT ,Human proteolytic subunit ELISA KIT , Human endopeptidase Clp ELISA KIT ,Human caseinolytic mitochondrial matrix peptidase proteolytic subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human putative atp-dependent clp protease proteolytic subunit, mitochondrial,CLPP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLPP, During the reaction, CLPP in the sample or standard competes with a fixed amount of biotin-labeled CLPP for sites on a pre-coated Monoclonal antibody specific to CLPP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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