Product Name:Human CMRF35- like molecule 8, CD300A ELISA KIT Packing:96T
Catalog No.:ELI-25610h
Gene Name:CD300A
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:CMRF35-like molecule 8(CD300A
Alternative Name:CD300A,CMRF35-like molecule 8(CD300A Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human CMRF35- like molecule 8, CD300A ELISA KIT allows for the in vitro quantitative determination of CMRF35-like molecule 8(CD300A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human CMRF35- like molecule 8, CD300A ELISA KIT has been pre-coated with an CMRF35-like molecule 8(CD300A antibody specific to CMRF35-like molecule 8(CD300A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CMRF35-like molecule 8(CD300A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CMRF35-like molecule 8(CD300A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of CMRF35-like molecule 8(CD300A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!