- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-25365h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Chromatin complexes subunit BAP18,BAP18
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Chromatin complexes subunit BAP18, BAP18 ELISA KIT
Product Name:Human Chromatin complexes subunit BAP18, BAP18 ELISA KIT
Packing:96T
Catalog No.:ELI-25365h
Gene Name:BAP18
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Chromatin complexes subunit BAP18(BAP18
Alternative Name:BAP18,Chromatin complexes subunit BAP18(BAP18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Chromatin complexes subunit BAP18, BAP18 ELISA KIT allows for the in vitro quantitative determination of Chromatin complexes subunit BAP18(BAP18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Chromatin complexes subunit BAP18, BAP18 ELISA KIT has been pre-coated with an Chromatin complexes subunit BAP18(BAP18 antibody specific to Chromatin complexes subunit BAP18(BAP18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Chromatin complexes subunit BAP18(BAP18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Chromatin complexes subunit BAP18(BAP18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Chromatin complexes subunit BAP18(BAP18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
carried over through protein-protein interactions. The protocol includes purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP
Use of In Vivo Biotinylation for Chromatin Immunoprecipitation
( protocol 1 ) ∼500‐bp fragments of sonicated E. coli DNA, optional (see protocol 1 , step 18) Takara Mighty Cloning Kit
INTRODUCTION This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach, the material is fixed with formaldehyde
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