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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Bcl-2-like protein 2,BCL2L2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Bcl- 2- like protein 2, BCL2L2 ELISA KIT
Product Name:Human Bcl- 2- like protein 2, BCL2L2 ELISA KIT
Packing:96T
Catalog No.:ELI-24456h
Gene Name:BCL2L2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Bcl-2-like protein 2(BCL2L2
Alternative Name:BCL2L2,Bcl-2-like protein 2(BCL2L2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Bcl- 2- like protein 2, BCL2L2 ELISA KIT allows for the in vitro quantitative determination of Bcl-2-like protein 2(BCL2L2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Bcl- 2- like protein 2, BCL2L2 ELISA KIT has been pre-coated with an Bcl-2-like protein 2(BCL2L2 antibody specific to Bcl-2-like protein 2(BCL2L2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bcl-2-like protein 2(BCL2L2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bcl-2-like protein 2(BCL2L2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bcl-2-like protein 2(BCL2L2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Immunodetecting Members of the Bcl-2 Family of Proteins
to a broad range of human diseases and disorders. Consequently, studying the regulation of Bcl-2 and its homologs has significant implications for biomedical research. The mechanisms by which Bcl-2 family members regulate apoptosis are complex, and include
Induction of Programmed Cell Death with an Antisense Bcl-2 Oligonucleotide
(3 ,4 ). Therefore, genetic manipulation of PCD regulatory genes may restore the altered homeostasis in these cells and permit these genetically damaged cells to enter PCD.A known regulator of the PCD pathway is Bcl-2, a protein capable of suppressing cell death
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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