- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-24270h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Monofunctional C1-tetrahydrofolate synthase,mitochondria
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Monofunctional C1- tetrahydrofolate synthase mitochondrial, MTHFD1L ELISA KIT 96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Monofunctional C1-tetrahydrofolate synthase mitochondrial,Formyltetrahydrofolate synthetase,C1TM, mitochondrial C1 tetrahydrofolate synthase,FTHFSDC1; MTC1THFS; dJ292B18.2; 10-formyl-THF synthetase; formyltetrahydrofolate synthetase domain containing 1; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Search name
Human Monofunctional C1-tetrahydrofolate synthase mitochondrial ELISA KIT ,Human Formyltetrahydrofolate synthetase ELISA KIT ,Human C1TM ELISA KIT ,Human mitochondrial C1 tetrahydrofolate synthase ELISA KIT ,Human FTHFSDC1 ELISA KIT ,Human MTC1THFS ELISA KIT ,Human dJ292B18.2 ELISA KIT ,Human 10-formyl-THF synthetase ELISA KIT ,Human formyltetrahydrofolate synthetase domain containing 1 ELISA KIT ,Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human monofunctional c1-tetrahydrofolate synthase, mitochondrial,C1TM concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1TM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C1TM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C1TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C1TM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Immunochemical Detection of Nitric Oxide Synthase in Human Tissue
To date three distinct isoforms of nitric oxide synthase (NOS) have been identified. Two isoforms are considered to be expressed constitutively—neu-ronal NOS (nNOS; type I NOS) and endothelial NOS (eNOS; type III NOS). The third isoform
Cloning and Expression of Human Inducible Nitric Oxide Synthase
NO synthase (NOS) pathway was first characterized and cloned in murine macrophages (1 –3 ). Activation with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) was uniformly used in these studies to increase inducible (i) NOS expression and improve cloning
ion-exchange, size-exclusion, and affinity chromatography, as well as the isolation of CLR and gC1q by limited enzymatic hydrolysis of the native C1q molecule. In this chapter, we described methods for purification of human C1q and its CLR and gC
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