Target Protein Name:Alkylglycerol monooxygenase(AGMO
Alternative Name:AGMO,Alkylglycerol monooxygenase(AGMO Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Alkylglycerol monooxygenase, AGMO ELISA KIT allows for the in vitro quantitative determination of Alkylglycerol monooxygenase(AGMO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Alkylglycerol monooxygenase, AGMO ELISA KIT has been pre-coated with an Alkylglycerol monooxygenase(AGMO antibody specific to Alkylglycerol monooxygenase(AGMO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Alkylglycerol monooxygenase(AGMO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alkylglycerol monooxygenase(AGMO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Alkylglycerol monooxygenase(AGMO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!