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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Kita-kyushu lung cancer antigen 1,KKLC1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Kita- kyushu lung cancer antigen 1, KKLC1 ELISA KIT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
KK LC 1,KKLC1; CT83; KK-LC-1; cancer/testis antigen 83; kita-kyushu lung cancer antigen 1; chromosome X open reading frame 61
Search name
Human KK LC 1 ELISA KIT ,Human KKLC1 ELISA KIT ,Human CT83 ELISA KIT ,Human KK-LC-1 ELISA KIT ,Human cancer/testis antigen 83 ELISA KIT ,Human kita-kyushu lung cancer antigen 1 ELISA KIT ,Human chromosome X open reading frame 61 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Kita- kyushu lung cancer antigen 1, KKLC1 ELISA KIT beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Kita- kyushu lung cancer antigen 1, KKLC1 ELISA KIT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Kita- kyushu lung cancer antigen 1, KKLC1 ELISA KIT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Kita- kyushu lung cancer antigen 1, KKLC1 ELISA KIT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Kita- kyushu lung cancer antigen 1, KKLC1 ELISA KIT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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【分享】Human press METHODS IN MOLECULAR BIOLOGY
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