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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
TRAF-interacting protein,TRAIP
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human TRAF- interacting protein, TRAIP ELISA KIT
Product Name:Human TRAF- interacting protein, TRAIP ELISA KIT
Packing:96T
Catalog No.:ELI-23139h
Gene Name:TRAIP
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:TRAF-interacting protein(TRAIP
Alternative Name:TRAIP,TRAF-interacting protein(TRAIP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TRAF- interacting protein, TRAIP ELISA KIT allows for the in vitro quantitative determination of TRAF-interacting protein(TRAIP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TRAF- interacting protein, TRAIP ELISA KIT has been pre-coated with an TRAF-interacting protein(TRAIP antibody specific to TRAF-interacting protein(TRAIP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TRAF-interacting protein(TRAIP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TRAF-interacting protein(TRAIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of TRAF-interacting protein(TRAIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验TRAF‐Mediated TNFR‐Family Signaling
, expression and chromosomal assignment of the ACT35 antigen. Eur. J. Immunol. 24:677‐683. Lee, S.Y., Lee, S.Y., and Choi, Y. 1997a. TRAF‐interacting protein (TRIP): A novel
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
pentagon, bait protein; open rectangle, noninteracting library protein; shaded box, activator protein (acid blob in Fig. ); open chevron, interacting library protein. View Image
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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