- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-23045h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
DNA mismatch repair protein Mlh3,MLH3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human DNA mismatch repair protein Mlh3, MLH3 ELISA KIT
Product Name:Human DNA mismatch repair protein Mlh3, MLH3 ELISA KIT
Packing:96T
Catalog No.:ELI-23045h
Gene Name:MLH3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:DNA mismatch repair protein Mlh3(MLH3
Alternative Name:MLH3,DNA mismatch repair protein Mlh3(MLH3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DNA mismatch repair protein Mlh3, MLH3 ELISA KIT allows for the in vitro quantitative determination of DNA mismatch repair protein Mlh3(MLH3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DNA mismatch repair protein Mlh3, MLH3 ELISA KIT has been pre-coated with an DNA mismatch repair protein Mlh3(MLH3 antibody specific to DNA mismatch repair protein Mlh3(MLH3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA mismatch repair protein Mlh3(MLH3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA mismatch repair protein Mlh3(MLH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA mismatch repair protein Mlh3(MLH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验In Vitro DNA Mismatch Repair in Human Cells
The in vitro DNA mismatch repair (MMR) assay is a very useful technique for studying the functions and the mechanisms of the MMR system in genome maintenance. This assay has been effectively used to evaluate MMR proficiency in various tumor
Immunostaining Human Paraffin-Embedded Sections for Mismatch Repair Proteins
The loss of the function in any one of four human DNA mismatch repair genes, hMSH2, hMLH1, hPMS1 , and hPMS2 , is thought to lead to deficient mismatch repair (MMR) of DNA in the somatic cells leading to increased mutations and thereby cancer
Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes
The detection of unknown mutations has proved complex and time consuming, and this is certainly the case for HNPCC. Germline mutations have been detected in five of the six human DNA mismatch repair genes (in hMLH1, hMSH2, hMSH6, hPMS1, hPMS2
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